Senior Research Associate

Senior Research Associate

Gilead

Amsterdam, Netherlands

Job Description

Under the supervision of a Scientist, the Senior Research Associate will independently perform, analyze and document experiments. Examples of the possible work tasks include, but are not limited to:

  • Isolation of primary human immune cells (T cells, B cells, Dendritic cells and other) from human blood and tumor material and subsequent aseptic cultivation;
  • Aseptic cultivation of (tumor) cell lines;
  • Evaluation of T cell populations for their characteristics, for example in terms of tumor reactivity using flow cytometry based assays (e.g. surface marker expression, intracellular cytokine staining, cytometric bead arrays);
  • Detection of T cell responses of interest in human donor material, for example with flow cytometry based assays;
  • Isolation of TCR candidate genes using single cell flow cytometry sorting and single-cell PCR;
  • Viral vector-based or electroporation based modification of tumor cell lines and primary human T cells;
  • Support in vivo studies conducted at third-party vendors;
  • Performing routine laboratory tasks such as genomic DNA/RNA isolation and PCR;
  • Taking charge of routine tasks relating to laboratory operations as assigned by the laboratory manager (e.g. organization and maintenance of lab supplies);
  • Other duties as assigned.

Requirements

  • A Bachelor (HLO) or Master in the area of Biomedical Sciences (or related) with at least 3 years of experience in the field of (tumor) immunology and T cell immunology is required;
  • Experience with cell culture aseptic techniques is required. Additional experience with multi-color flow cytometry is an advantage;
  • Hands-on experience in any of the following areas is a plus: flow cytometry cell sorting, molecular biology (in particular, single-cell based PCR methods such as droplet-PCR), tumor animal models, genetic screens with immune cells, cell purification methods (e.g. MACS);
  • Strong verbal and written communication skills in English and ability to communicate results to a diverse cross-functional audience is required;
  • Proactive, self-motivated team player with strong interpersonal skills, appropriate sense of urgency and the ability to work independently is a must;
  • Ability to work in a fast-paced environment with challenging work-load and to quickly get acquainted with novel methods and approaches is required.

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© EuroJobsites 2018

EuroJobsites is a UK registered company number: 4694396 VAT number: GB 880 9055 04

Registered address: EuroJobsites Ltd, Unit 8, Kingsmill Business Park, Kingston Upon Thames, London, KT1 3GZ, United Kingdom

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